Bin space is typically limited for multi-species feed mills; therefore, ingredient inventory is prioritized based on species specialization. The objective of this study was to determine if replacing solvent-extracted soybean meal (SBM) with an extra heat-treated extruded soybean meal (ESBM) intended as a source of by-pass protein for ruminants is suitable for broiler chickens. We hypothesize that ESBM would decrease broiler performance, as amino acid digestibility is expected to be reduced because additional heat treatment could have caused Maillard reactions. A total of 1,120 one-day-old female Ross 708 broiler chicks were randomly allocated to 28 floor pens of 40 chicks each. Each pen was 3.99 m2 with fresh pine shaving as bedding. Pens were assigned to 1 of 4 treatments (n=7 pens/treatment) in a randomized complete block design with pen location as blocking factor. Treatments were 0% ESBM (typical commercial broiler diets in Ontario, Canada with SBM as the main protein source), 33% ESBM, 66% ESBM and 100% ESBM, with ESBM replacing SBM at the respective levels. Diets were formulated with ME value within 0.1% of the control diet and the first 3 limiting digestible amino acids within 1.5% of the control diet. Feed and water were provided ad libitum. Pen body weight and feed intake were determined weekly to day 42, and mortality-corrected feed conversion ratio was calculated. Feed cost was calculated using ingredient prices at the time of the experiment. Price of the resulting chicken meat was determined at processing to calculate income over feed cost (IOFC). At day 28, weights of the proventriculus, gizzard, and pancreas from two birds per pen were measured; cecal digesta was pooled into one sample per pen. Performance and economic traits were analyzed as a one-way ANOVA using pen as the experimental unit and regression analysis using R 4.2.1. At 21, 28, 35 and 42 days, BW was greater (P < 0.05) for broilers fed 0, 33, or 66% ESBM than those fed 100% ESBM. At all periods except 35 to 42 days, FCR was lower (P < 0.05) for broilers fed 0, 33, or 66% ESBM than those fed 100% ESBM. From 29 to 35 days, the 100% ESBM group had the lowest FI and the 0% ESBM treatment had the highest. Organ weights and relative SCFA content did not differ among treatments. The 33% ESBM had the second-lowest total cost (P < 0.05), the lowest feed cost per kg gained (P < 0.05) and the highest IOFC (P < 0.05).